| 质粒名称: | pLenti CMV GFP Blast (659-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17445 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7603 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 杀稻瘟菌素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP,enhanced GFP |
| 物种来源: | -- |
| 插入大小: | 719 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | Sal I(未破坏) |
| 5' 测序引物 | CMVforw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV GFP Blast (659-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17445 ; http://n2t.net/addgene:17445 ; RRID:Addgene_17445) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
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pLenti CMV GFP Blast (659-1)载体质粒基本信息
pLenti CMV GFP Blast (659-1)质粒图谱载体图谱和pLenti CMV GFP Blast (659-1)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pLenti CMV GFP Blast (659-1)质粒载体简介
pLenti CMV GFP Blast (659-1)质粒序列载体序列
LOCUS pLenti CMV GFP Blast (659-1) 8311 bp ds-DNA circular SYN 03-MAY-2019
DEFINITION 3rd gen lentiviral eGFP expression vector, CMV promoter, Blast.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV GFP Blast (659-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8311)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 8311)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 3, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8311
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 26..130
/gene="bla"
/label=AmpR promoter
CDS 131..991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(349..368)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1162..1750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1651..1670
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1904..1921
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2038..2059
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2074..2104
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2112..2128
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2117..2139
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2136..2152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2136..2152
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2171..2191
/label=T3
/note="T3 promoter, forward primer"
promoter 2173..2191
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2219..2445
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 2446..2626
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2673..2798
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3291..3524
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4020..4136
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4159..4462
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4463..4666
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4617..4637
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4663..4687
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 4756..4765
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4762..5481
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4807..4828)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5068..5087)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5415..5436
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5497..6085
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5550..5570)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5968..5979)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6136..6156)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6141..6470
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6321..6456
/label=SV40 ori
/note="SV40 origin of replication"
promoter 6518..6565
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6584..6982
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
LTR 7069..7302
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7374..7495
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7411..7430)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7465..7484
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7514..7649
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(7669..7688)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7670..7688)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7698..7715)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7698..7714)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7707..7729)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7856..8311
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7943..7962)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8153..8174
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
4741 gaggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
4801 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
4861 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
4921 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
4981 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
5041 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
5101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
5161 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
5221 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
5281 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
5341 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
5401 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
5461 atggacgagc tgtacaagta aagcggccgc gtcgacaatc aacctctgga ttacaaaatt
5521 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5581 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
5641 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
5701 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
5761 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
5821 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
5881 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
5941 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
6001 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6061 atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggtta gtaatgagtt
6121 tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
6181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
6481 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
6541 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
6601 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
6661 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
6721 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
6781 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
6841 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
6901 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
6961 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
7021 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
7081 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
7141 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
7201 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
7261 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
7321 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
7381 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
7441 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
7501 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
7561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
7621 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
7681 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
7741 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
7801 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
7861 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
7921 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
7981 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
8041 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
8101 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
8161 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
8221 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
8281 aattttaaca aaatattaac gcttacaatt t
//
